OxyFile #541

TI:  Weekly Repeated Therapeutical 10000 ug Venous Blood 
     Ozonations.  Evaluation of Lipid Peroxydation by the way of 
     Bilirubin, Malondialdehyd, Superoxyd Dismutase, and 
     Glutathion Peroxydase measurements: A Study about 178 
     Comparative Controls

DT:  September 1993

AU:  J.-M. Winkler M.D., 13, rue de l'eglise, 25660 More (France)

AB:  Ozone, an allotropic form of oxygne, has to be administrated 
     by the way of extracorporal venous blood ozonation every 
     time, when masses such as 10000y have to be used.  The does 
     has to be chosen in order to insure therapeutical efficiency 
     and avoid as far as possible any toxicity.  These limits are 
     lying close together for oxygen as for ozone.  Ozone 
     degradation produces free radicals, the peroxydation of 
     fatty acids and the increase of Malondialdehyd (MDA).  
     Several safety systems, either enzymatic such as Superoxyd 
     dismutase (SOD) and Glutathion peroxydase (GPX) or non 
     enzymatic as Vitamin E, Vitamin A, and indirectly Vitamin C, 
     have to insure the protection of the organism against 
     toxical effects.  MDA increases after a single ozonation 
     with 10000y  of 15%, GPX and SOD underly no statisticaly 
     significant variation.  After repetition of this treatment 
     each week, no variations of MDA, SOD or GPX are noticed at 
     the end of the treatments while intermediary results are 
     founded after the first group of ten treatments: MDA + 4.17% 
     (N.S.), SOD no variation.  These surprising results induct 
     the question of a possible adaptation to an high but to a 
     short time limited oxydative stress.  These results should 
     be compared with those of other randomized studies and 
     completed with other clinical trials performed using 
     different ozone masses and different ozone concentrations.