OxyFile #424

Influence of ozone on haemoglobin oxygen affinity in type-2 
diabetic patients with peripheral vascular disease: in vitro 

Coppola L; Giunta R; Verrazzo G; Luongo C; Sammartino A; Vicario 
C; Giugliano D

Department of Gerontology, Geriatrics and Metabolic Diseases, 
Second University of Naples, Italy.

Diabete Metab, 21: 4, 1995 Oct, 252-5


The use of ozone in the treatment of peripheral vascular disease 
(PVD) is increasing. The purpose of this study was to evaluate the 
effect of ozone on haemoglobin oxygen affinity in Type-2 diabetic 
patients with PVD. Twenty diabetic patients presenting with PVD 
(Clinical stage II-IV according to Fontaine) and 20 non-diabetic 
healthy matched subjects were studied. In both groups, aliquots of 
blood were ozonised with mixtures of oxygen-ozone (O2-O3) to reach 
end-concentrations of 6.5, 13, 26 and 78 micrograms O3 per ml of 
substrate. At baseline, diabetic patients presented significantly 
lower haemoglobin oxygen affinity values but higher plasma levels 
of free haemoglobin and malonyldialdehyde (MDA) than controls. In 
both diabetic patients and controls, exposure of blood to ozone 
reduced haemoglobin oxygen affinity in an "all-or-none" fashion, 
without changing 2-3, diphosphoglycerate concentrations in 
erythrocytes. Both free haemoglobin and MDA concentrations showed 
significant, dose-dependent increases after blood ozonisation. 
Thus, ozone caused a significant increase in oxygen unloading of 
haemoglobin in both normal subjects and Type-2 diabetic patients 
with PVD.