Studies on the biological effects of ozone: 6. Production of transforming growth factor 1 by human blood after ozone treatment. Bocci V; Luzzi E; Corradeschi F; Silvestri S Institute of General Physiology and Nutritional Sciences, Siena, Italy. J Biol Regul Homeost Agents, 8: 4, 1994 Oct-Dec, 108-12 Abstract After exposing human whole blood from normal volunteers to ozone concentrations ranging from 22 to 156 micrograms/ml, we have shown that, upon incubation of up to 8 hours, there is a significant release of transforming growth factor beta (TGF-beta 1). In comparison to TGF-beta 1, TGF-beta 2 production is not influenced by ozone concentrations. In line with our previous findings it appears that blood, in the presence of heparin and 5mM Ca,2+ allows a consistent production of tumor necrosis factor a (TNF alpha) and the release of low and non-hazardous levels of free hemoglobin. These data support the contention that autohemotherapy performed after treating blood with ozone followed by reinfusion into the donor, may represent a valuable therapeutic approach for achieving immunoregulatory effects.