OxyFile #384

This article is apart of a series. Please read the preface (oxy00362) for more details.

Authority: Hans Kleinmann, M.D.
German Research Authority


Subject:  OZONE - Its action on Surface Cultures, Bact. coli -
Bacilli Diphtheria, Staphylococcus and Streptococcus


Action of Ozone on Bact. coli 2 hours after vaccination.

Spread: 0.3 ccm solution  1:50000 of a 24 hour bouillon culture
upon Endo medium.  Two hours air dried, ozonized without pressure.

Ozone            Time of       Bact. count        Mortification
Concentration    ozonation     after 36 hrs.      Percent
mg./1 hr         Minutes       incubation
                               colonies

 174.3             0            Ca 2-3000             0
 174.3             2               60                 98
 174.3             8               15                 99.5


Action of Ozone on surface cultures of Diphtheria Bacilli four
hours after vaccination.

0.5 solution 1-50000 of a 48 hour bouillon culture on serum
plates.  Two hours.  Air dried after two hours.  Ozonized.

Ozone            Time of       Bact. count        Mortification
Concentration    ozonation     after 24 hrs.      Percent
mg./1 hr         Minutes       incubation
                               colonies

 174.3              0              1126               0
 174.3              2                 0               98.1
 174.3              8                 0               100


Action of Ozone on Staphylococcus, Pyogens Aureus, four hours
alter vaccination.

0.3 ccm solution 1010000 of a 24 hour bouillon culture spread on
agar plates.  Dry one hour.  Ozonized after three hours.


Ozone            Time of       Bact. count        Mortification
Concentration    ozonation     after 24 hrs.      Percent
mg./1 hr         Minutes       incubation
                               colonies

 174.3              0              840               98
 174.32             0                0              100

In "Action of Ozone on Pathogenic Germs"


Action of Ozone on non-hemolylic streptococcus, four hours after
vaccination.

0.5 ccm solution 1-10000 of a 48 hour bouillon culture of blood
agar plates.  Two hours.  Dried after two hours of ozonation.


Ozone            Time of       Bact. count        Mortification
Concentration    ozonation     after 24 hrs.      Percent
mg./1 hr         Minutes       incubation
                               colonies

 174.3              0            Ca 2000              0
 174.3              2            Steril             100


All these tables testify that Ozone, very quickly and
energetically, acts deadly on germs, grown macroscopically and
dried on a medium soil.

The first table shows a mortification of 98% and 99% of the bact.
coli within two minutes and this confirms completely the results
of Dr. Heise.  It also gave evidence that the action of Ozone is
very intense on the plates and grows six (6) hours after the
vaccination.

The other plates, dysentry, streptococcus, staphylococcus, which
commonly were ozonized after 3-4 hours after vaccination were
absolute steril alter two (2) minutes.

According to the results of these experiments as shown in these
tables, the disinfectory germical action of Ozone must be
considered as most excellent and superior to other methods.

In "Action of Ozone on Pathogenic Germs"