OxyFile #360

Hyperbaric oxygen reduces ischemia-induced skeletal muscle injury.

Haapaniemi T; Nylander G; Sirsjš A; Larsson J

Department of Plastic Surgery, Hand Surgery, and Burns, Clinical 
Research Center, Faculty of Health Sciences, University Hospital, 
Linkoping, Sweden.

Plast Reconstr Surg, 97: 3, 1996 Mar, 602-7; discussion 608-9


The effect of hyperbaric oxygen treatment on skeletal muscle 
submitted to 3 or 4 hours of ischemia was studied in a rat 
hindlimb model after 48 hours of reperfusion. Forty-eight male 
Sprague-Dawley rats were allocated to four groups. In the two 
treatment groups, hyperbaric oxygen was given for 45 minutes at 
2.2 atm immediately and 4,8,16,24,32, and 40 hours after release 
of the ischemia-inducing tourniquet. The injury to skeletal muscle 
was quantified from the uptake of 99mtechnetium-pyrophosphate 
(injected intravenously after 45 hours of reperfusion) in anterior 
tibial muscle harvested 3 hours later. The uptake was 
significantly lower in hyperbaric oxygen-treated rats than in 
untreated rats with 3 or 4 hours of ischemia (p < 0.01 and P < 
0.05). After 4 hours of ischemia, the changes in levels of the 
intracellular muscle compounds adenosine triphosphate, 
phosphocreatine, and lactate were less in the hyperbaric oxygen-
treated rats than in the untreated animals.