Therapeutical Significance of Intestinal Insufflation of Medical Ozone Dr. med. Horst Kief, Ludwigshafen Vasti is the name of a special treatment: for this treatment, make the patient sit down in water the level of which reaches up to the patient's navel. Then introduce a tube into the patient's rectum and flush with water. Such type of purgation is called Vasti. Vasti will heal intestinal diseases, splenalgia and stomach disorder, and will remedy all diseases connected to the respiratory system, bile and juices. This type of enterocleaning will regenerate your body, senses and organs. It will give you splendour, improve digestion and remove all kind of trouble. (HATHA-YOGA-PRADIPIKA, APPROX. 1400 AFTER CHRIST, VERSES 26, 27, 28) The application of ozone in medical therapy is decisively restricted by the fact that the ozone in its form of activated oxygen cannot be supplied to the lung being the organ destined for feeding oxygen to the human organism due to the specific toxicity of ozone. It is true that the clinical experiments aimed at trying minimum concentrations are based on a reasonable theoretic approach (1) but are thwarted by legal regulations mainly based on the threshold limit value of 0.4 ppm. Any uptake of ozone through the skin is impossible, since the ozone will react with boundary layers immediately. Generally, physicians getting into the subject for the first time are amazed by the extraordinary range of application of ozone in parenteral application, although there is a strongly emotional rejection of ozone in respect of parenteral application. which, however, is absolutely unfounded due to scientific findings and clinical experience. When using ozone in parenteral application, one should be perfectly aware of the fact that puncture of the vein and intramuscular and subcutaneous injection constitutes a bodily injury before law, therefore, people are rightly looking for ever new approaches to prevent such type of therapy. In this respect, the intestinum with its enormous surface of resorption is the obvious choice of application. Moreover, therapeutical effects similar to those of parenteral application such as Hyperbaric Medical Ozone therapy, may reasonably be expected due to the volume of the 02/03-compound which can be insufflated into the lumen of the rectal tube owing to the special anatomic features of the colon. In this connection, the particularities of this organ should be highlighted: 1. The intestinum of adults is approx. 12 m long and due to the multitude of intestinal villi and crypts features a surface of 10 square meters. 2. The lymphatic system of the intestinum, i.e. Peyer's patches, occupy more space within the human organism than is used by the spleen and the thymus together. 3. The circulatory system of the intestinum is directly connected to the liver through the portal vein, where most of the biochemical, immunological and metabolic processes take place. 4. "Death lives in the intestines" - this popular saying presumably is derived from the deeply rooted archaic knowledge that the intestinal flora - the pathological flora in particular - may trigger a number of diseases, which most frequently is misjudged on rather underestimated by traditional medicine. Considering the anatomic, immunological, biochemical and microbiological particularities of the intestinum, the use of Medical Ozone should may reasonably be expected to provide decisive therapeutical consequences in rectal application, and to yield successful clinical results. The use of Medical Ozone in rectal application ad 1. should provide for measurable resorption to be obtained upon insufflation of sufficient volumes. ad 2, should provide for a substantial immunological effect to be obtained due to direct contamination of the largest lymphatic system of the human organism. ad 3. should yield improvements relating to all oxidative processes taking place in the liver due to the increased flood of reactive oxygen metabolites supplied to this organ. ad 4. should provide for the microbiological milieu to be improved in cases of pathological changes of the intestinal flora due to old age and wrongful eating habits within our society. The increase in the partial pressure of oxygen following rectal application of an O2/O3-compound has been established repeatedly. Further details were supplied by Knoch by way of animal experiments 3, 4). The same author furnished proof of the effectiveness of rectal application of Medical Ozone in cases of hepatitis, within the framework of a controlled study (5). Although various evidence has been provided concerning the bactericidal effect of ozone on different species of bacteria in aqueous environments (6-10), there haven't been any studies investigating the direct effect of O2/O3-compounds on the intestinal flora in man, so far. It is true that the rectal application of O2/O3-compounds was reported to be successful in cases of juvenile neurodermititis (11), but there hasn't been any direct proof furnished so far, despite the fact that generally there is a connection established between unphysiologic intestinal flora and neurodermititis and that the effectiveness of such type of application for this indication may reasonably be presumed to result from the mechanism described herein. This is particularly true for fungi presumed to be one of the causes of neurodermititis, the quantity of which appears not to be affected by O2/O3-compounds in the intestinum (11). It may, however, be reasonably assumed that the reason for this lies in that the gas is insufflated in the intestinum which is filled with excrements, and therefore cannot reach all of the pathogenic flora, which is particularly true for the fungi settled in-between the intestinal villi and in the crypts. One of the prerequisites to remedy such deficiency consists in purgation using irrigators prior to Medical Ozone insufflation. It is perfectly clear that the resorption of the O3-compound will be much better in the case of purged Intestinal walls than the one provided by intestinal insufflation in the case of intestines filled with excrements or to the walls of which faecal residues may adhere in addition. Apart from that, it is perfectly clear that the O2/O3-compound is enabled to rise much higher in the lumen with the intestinum purged before, and to contaminate areas of the microflora, which would not be reached by the gas flow without preliminary purgation. In addition to the benefits described above offered by the alternating use of intestinal irrigation and Medical Ozone insufflation, the combined water MEDICAL OZONE irrigation uses freshly ozonied water. It was yielded from measurements of the redox potential using water of various qualities that the redox potential obtained for freshly ozonized water by far exceeds the one obtained for untreated water. Materials and Methods: 20 ml of O2/O3-compound were added to 10 ml of water resulting in a concentration of 24 µg/ml. The water was stored in a 50 ml test tube and after ozonization was agitated thoroughly. The 3 water qualities listed in the following were used: 1. aqua bidestillata in the form of "Ampuva", injectable water. 2. town water (supplied by the waterworks of Ludwigshafen) 3. town water (of the same origin) gained from counterosmosis and filtered. For determining the redox potential, measurement was first performed for the blank and then for the redox potential of pretreated water after 5, 10, 15, 20, 25, 30, 60. 90 and 120 minutes, following basic calibration against ninhydrin. A number of 6 measurements were performed for each time interval and for each water quality. Following this . the median value and the standard deviation were determined for each series of measurement. The pO2 was measured following blank determination after 5, 30, 60, 90 and 120 minutes from addition of the O2/O3-compound featuring the same concentration. Equipment used: Measurement of the redox potential was performed using an Ingold- brand platinum electrode, serial number:type PT61, and pH- electrode type N61, and the pH- and redox potential measuring unit type CG817 of Schott GerŠte GmbH, D-623B Hofheim/Taunus. pO2-measurement was performed using a Kontron-brand pO2-monitor type IN 632. For ozone generation the Biozomat unit type 12T of Clinico in Bad Hersfeld was used. The blanks obtained for the redox potentials of the three water qualities (aqua bidest, town water and town water filtrate gained from counterosmosis) are very close and range at plus 200 mv (table 1 and diagrams 1-3) . The absorbing capacity of the three water qualities is highly different due to the ozone induced oxidation potential. The highest values were obtained for aqua bidest, followed by town water. The lowest values were obtained for town water filtrate gained from counter osmosis. While the town water and aqua bidest loose about two thirds of their capacity over a period of 2 hours, the town water filtrate gained from counterosmosis. While the town water and aqua bidest loose about two thirds of their capacity over a period of 2 hours, the town water filtrate gained from counterosmosis will loose only half of its absorbing capacity over the same period (Diagram 4). The pH-values obtained for the three different water qualities used were as follows: Water quality pH prior to 03, after 03, Ampuva 6.91 7.00 Town water 7.65 7.80 Counterosmosls 7.20 7.35 filtrate It may be seen from this that Ampuva provides for the most neutral results and will maintain its neutral status while the town water and the counterosmosis filtrate react alkaline to ozone and after ozonization clearly tend to become more alkaline. In town water. the decrease in pH is 0.15, in counterosmosis filtrate is 0.33 and in Ampuva is as little as 0.9g. Resulting from this is that the loss of protons is highest for the counterosmosis filtrate, followed by town water. while Ampuva proved to react almost neutrally. The loss of protons observed for the relatively neutral water qualities after ozonization may reasonably be explained by the transformation into water caused by the release of an oxygen radical from the ozone molecule according to the formula 2 H(+) + 1 O2 = H2O The pO2-blanks determined for the three water qualities all range at approx. 150 mm Hg (exact values from 153 to 157 mm Hg) depending on the partial pressure of oxygen prevailing (table 2). Although the redox potentials of the three water qualities are very different, their saturation with physically dissolved oxygen is almost the same (diagrams 5. 6 and 7). Both, the town water and the counterosmosis filtrate are equally ready to release the oxygen to the environment. Such readiness is relatively low for aqua bidest which will release as little as 50 mm Hg to the environment over a comparable period of 120 minutes (diagram 8). It stands to reason that the use of water which was pretreated in this way per se will provide for a therapeutical effect on the intestinal flora and on the physiology of the entire milieu . It surely is significant that the water used for irrigation is cleaned before use, since the town water supplied is not pure, i.e. germ-free and free of suspended matter, as generally believed (12) . This is why we used town water which was purified by means of counterosmosis for our own trials. In practical applications, the combined method of intestinal irrigation/Medical Ozone insufflation did not cause any problems. Especially, it showed that the alternating use of irrigation and insufflation removed residual excrements and faeces from intestinal sections located at higher levels, which was not observed when losing one of the two therapies alone. It is, however hard to draw up the list of indications for such combination of treatment, which is not because indications are lacking, but rather because the intestinum on the one hand is such a significant organ and on the other hand is neglected frequently enough. Consequently, the colozone therapy is justified as basic treatment in almost any case. This is particularly true for indications such as colostatis acute and chronic hepatitis disturbed bacterial flora atonia and dystonia of the gastrointestinal system colitis mucosa et ulcerosa Crohn's disease while the use of such treatment in cases of migrain polyarthritis eczemas asthma and acne may not be evident to physicians, unless aquainted with the matter and the specific physiological correlations involved. The use of colozone therapy is highly recommended Reference: available from the author.