OxyFile #350


Therapeutical Significance of Intestinal Insufflation of Medical 

Dr. med. Horst Kief, Ludwigshafen

Vasti is the name of a special treatment: for this treatment, make 
the patient sit down in water the level of which reaches up to the 
patient's navel.  Then introduce a tube into the patient's rectum 
and flush with water.  Such type of purgation is called Vasti.

Vasti will heal intestinal diseases, splenalgia and stomach 
disorder, and will remedy all diseases connected to the 
respiratory system, bile and juices.

This type of enterocleaning will regenerate your body, senses and 
organs.  It will give you splendour, improve digestion and remove 
all kind of trouble.


The application of ozone in medical therapy is decisively 
restricted by the fact that the ozone in its form of activated 
oxygen cannot be supplied to the lung being the organ destined for 
feeding oxygen to the human organism due to the specific toxicity 
of ozone.  It is true that the clinical experiments aimed at 
trying minimum concentrations are based on a reasonable theoretic 
approach (1) but are thwarted by legal regulations mainly based on 
the threshold limit value of 0.4 ppm.  Any uptake of ozone through 
the skin is impossible, since the ozone will react with boundary 
layers immediately.

Generally, physicians getting into the subject for the first time 
are amazed by the extraordinary range of application of ozone in 
parenteral application, although there is a strongly emotional 
rejection of ozone in respect of parenteral application. which, 
however, is absolutely unfounded due to scientific findings and 
clinical experience.

When using ozone in parenteral application, one should be 
perfectly aware of the fact that puncture of the vein and 
intramuscular and subcutaneous injection constitutes a bodily 
injury before law, therefore, people are rightly looking for ever 
new approaches to prevent such type of therapy.  In this respect, 
the intestinum with its enormous surface of resorption is the 
obvious choice of application.  Moreover, therapeutical effects 
similar to those of parenteral application such as Hyperbaric 
Medical Ozone therapy, may reasonably be expected due to the 
volume of the 02/03-compound which can be insufflated into the 
lumen of the rectal tube owing to the special anatomic features of 
the colon. 

In this connection, the particularities of this organ should be 

1. The intestinum of adults is approx. 12 m long and due to the 
multitude of intestinal villi and crypts features a surface of 10 
square meters.

2. The lymphatic system of the intestinum, i.e. Peyer's patches, 
occupy more space within the human organism than is used by the 
spleen and the thymus together.
3. The circulatory system of the intestinum is directly connected 
to the liver through the portal vein, where most of the 
biochemical, immunological and metabolic processes take place. 

4. "Death lives in the intestines" - this popular saying 
presumably is derived from the deeply rooted archaic knowledge 
that the intestinal flora - the pathological flora in particular - 
may trigger a number of diseases, which most frequently is 
misjudged on rather underestimated by traditional medicine.  
Considering the anatomic, immunological, biochemical and 
microbiological particularities of the intestinum, the use of 
Medical Ozone should may reasonably be expected to provide 
decisive therapeutical consequences in rectal application, and to 
yield successful clinical results.  The use of Medical Ozone in 
rectal application 

ad 1. should provide for measurable resorption to be obtained upon 
insufflation of sufficient volumes. 

ad 2, should provide for a substantial immunological effect to be 
obtained due to direct contamination of the largest lymphatic 
system of the human organism. 

ad 3. should yield improvements relating to all oxidative 
processes taking place in the liver due to the increased flood of 
reactive oxygen metabolites supplied to this organ. 

ad 4. should provide for the microbiological milieu to be improved 
in cases of pathological changes of the intestinal flora due to 
old age and wrongful eating habits within our society.
The increase in the partial pressure of oxygen following rectal 
application of an O2/O3-compound has been established repeatedly.  
Further details were supplied by Knoch by way of animal 
experiments 3, 4).

The same author furnished proof of the effectiveness of rectal 
application of Medical Ozone in cases of hepatitis, within the 
framework of a controlled study (5).

Although various evidence has been provided concerning the 
bactericidal effect of ozone on different species of bacteria in 
aqueous environments (6-10), there haven't been any studies 
investigating the direct effect of O2/O3-compounds on the 
intestinal flora in man, so far. It is true that the rectal 
application of O2/O3-compounds was reported to be successful in 
cases of juvenile neurodermititis (11), but there hasn't been any 
direct proof furnished so far, despite the fact that generally 
there is a connection established between unphysiologic intestinal 
flora and neurodermititis and that the effectiveness of such type 
of application for this indication may reasonably be presumed to 
result from the mechanism described herein.  This is particularly 
true for fungi presumed to be one of the causes of 
neurodermititis, the quantity of which appears not to be affected 
by O2/O3-compounds in the intestinum (11).  It may, however, be 
reasonably assumed that the reason for this lies in that the gas 
is insufflated in the intestinum which is filled with excrements, 
and therefore cannot reach all of the pathogenic flora, which is 
particularly true for the fungi settled in-between the intestinal 
villi and in the crypts.  One of the prerequisites to remedy such 
deficiency consists in purgation using irrigators prior to Medical 
Ozone insufflation.  It is perfectly clear that the resorption of 
the O3-compound will be much better in the case of purged 
Intestinal walls than the one provided by intestinal insufflation 
in the case of intestines filled with excrements or to the walls 
of which faecal residues may adhere in addition.  Apart from that, 
it is perfectly clear that the O2/O3-compound is enabled to rise 
much higher in the lumen with the intestinum purged before, and to 
contaminate areas of the microflora, which would not be reached by 
the gas flow without preliminary purgation.

In addition to the benefits described above offered by the 
alternating use of intestinal irrigation and Medical Ozone 
insufflation, the combined water MEDICAL OZONE irrigation uses 
freshly ozonied water. It was yielded from measurements of the 
redox potential using water of various qualities that the redox 
potential obtained for freshly ozonized water by far exceeds the 
one obtained for untreated water.

Materials and Methods: 

20 ml of O2/O3-compound were added to 10 ml of water resulting in 
a concentration of 24 µg/ml.  The water was stored in a 50 ml test 
tube and after ozonization was agitated thoroughly. The 3 water 
qualities listed in the following were used: 

1. aqua bidestillata in the form of "Ampuva", injectable water. 
2. town water (supplied by the waterworks of Ludwigshafen) 
3. town water (of the same origin) gained from counterosmosis and 

For determining the redox potential, measurement was first 
performed for the blank and then for the redox potential of 
pretreated water after 5, 10, 15, 20, 25, 30, 60. 90 and 120 
minutes, following basic calibration against ninhydrin. A number 
of 6 measurements were performed for each time interval and for 
each water quality. Following this . the median value and the 
standard deviation were determined for each series of measurement. 
The pO2 was measured following blank determination after 5, 30, 
60, 90 and 120 minutes from addition of the O2/O3-compound 
featuring the same concentration. 

Equipment used: 

Measurement of the redox potential was performed using an Ingold-
brand platinum electrode, serial number:type PT61, and pH-
electrode type N61, and the pH- and redox potential measuring unit 
type CG817 of Schott GerŠte GmbH, D-623B Hofheim/Taunus.

pO2-measurement was performed using a Kontron-brand pO2-monitor 
type IN 632. For ozone generation the Biozomat unit type 12T of 
Clinico in Bad Hersfeld was used.
The blanks obtained for the redox potentials of the three water 
qualities (aqua bidest, town water and town water filtrate gained 
from counterosmosis) are very close and range at plus 200 mv 
(table 1 and diagrams 1-3) . The absorbing capacity of the three 
water qualities is highly different due to the ozone induced 
oxidation potential. The highest values were obtained for aqua 
bidest, followed by town water. The lowest values were obtained 
for town water filtrate gained from counter osmosis. While the 
town water and aqua bidest loose about two thirds of their 
capacity over a period of 2 hours, the town water filtrate gained 
from counterosmosis. While the town water and aqua bidest loose 
about two thirds of their capacity over a period of 2 hours, the 
town water filtrate gained from counterosmosis will loose only 
half of its absorbing capacity over the same period (Diagram 4). 

The pH-values obtained for the three different water qualities 
used were as follows:
Water quality pH prior to 03, after 03, 
  Ampuva            6.91          7.00 
  Town water        7.65          7.80 
  Counterosmosls    7.20          7.35

It may be seen from this that Ampuva provides for the most neutral 
results and will maintain its neutral status while the town water 
and the counterosmosis filtrate react alkaline to ozone and after 
ozonization clearly tend to become more alkaline. In town water. 
the decrease in pH is 0.15, in counterosmosis filtrate is 0.33 and 
in Ampuva is as little as 0.9g. Resulting from this is that the 
loss of protons is highest for the counterosmosis filtrate, 
followed by town water. while Ampuva proved to react almost 
neutrally. The loss of protons observed for the relatively neutral 
water qualities after ozonization may reasonably be explained by 
the transformation into water caused by the release of an oxygen 
radical from the ozone molecule according to the formula 

 2 H(+) + 1 O2 = H2O

The pO2-blanks determined for the three water qualities all range 
at approx. 150 mm Hg (exact values from 153 to 157 mm Hg) 
depending on the partial pressure of oxygen prevailing (table 2). 
Although the redox potentials of the three water qualities are 
very different, their saturation with physically dissolved oxygen 
is almost the same (diagrams 5. 6 and 7). Both, the town water and 
the counterosmosis filtrate are equally ready to release the 
oxygen to the environment. Such readiness is relatively low for 
aqua bidest which will release as little as 50 mm Hg to the 
environment over a comparable period of 120 minutes (diagram 8).  

It stands to reason that the use of water which was pretreated in 
this way per se will provide for a therapeutical effect on the 
intestinal flora and on the physiology of the entire milieu . It 
surely is significant that the water used for irrigation is 
cleaned before use, since the town water supplied is not pure, 
i.e. germ-free and free of suspended matter, as generally believed 
(12) . This is why we used town water which was purified by means 
of counterosmosis for our own trials. 

In practical applications, the combined method of intestinal 
irrigation/Medical Ozone insufflation did not cause any problems. 
Especially, it showed that the alternating use of irrigation and 
insufflation removed residual excrements and faeces from 
intestinal sections located at higher levels, which was not 
observed when losing one of the two therapies alone.
It is, however hard to draw up the list of indications for such 
combination of treatment, which is not because indications are 
lacking, but rather because the intestinum on the one hand is such 
a significant organ and on the other hand is neglected frequently 
enough. Consequently, the colozone therapy is justified as basic 
treatment in almost any case. This is particularly true for 
indications such as 

acute and chronic hepatitis 
disturbed bacterial flora 
atonia and dystonia of the gastrointestinal system 
colitis mucosa et ulcerosa 
Crohn's  disease 

while the use of such treatment in cases of 

and acne 

may not be evident to physicians, unless aquainted with the matter 
and the specific physiological correlations involved. 

The use of colozone therapy is highly recommended 

Reference: available from the author.