OxyFile #293

Animal Models for the Application of Ozone in Veterinary and Human 

S. Schulz
Tierarztlicher Dienst der Philipps-Universitat Marburg

The use of animals in medical research is one of the basic principles 
used in order to increase our knowledge of the pathogenesis and 
alleviation of the symptoms of diseases in both animals and humans.

Therapeutic experiments on animals with induced diseases can be 
objectively better controlled than is the case with human patients.

In toxicological ozone research, many different animals species have 
been used in more than 100 years of ozone science.  However, in 
experimental studies with ozone, no or very few animals models have 
been used.  Questions arise as to the reasons for this.  Do the 
answers perhaps lie in an attempt to save animals in line with the 
views of the animal protectionists, or is it simply the lack of 
suitable animal models, or worse still, a blissful ignorance 
regarding certain basic principles in medical science?

Tab. 1 shows a survey of referates from the first Iberoamerican 
Congress in Cuba (1990) on Ozone application, with a caleidoscope of 
diseases in man treated with ozone or ozonized products.  
Unfortunately, but rather typical, there are no results of 
experiments on suitable animal models.  This situation demonstrates 
the dilemma in ozone therapy.  Scientists in Cuba have to experiment 
with cheap, alternative therapies directly on man, mainly because of 
the economic isolation due to the U.S. trade embargo and the absence 
of competitive pharmaceutical firms.  Sunflower oil and its ozonised 
compound is a cheap and effective product with no risks on external 
application.  However, the application with ozone gas mixtures, with 
possible therapeutic effects and unwanted side-effects within the 
broad spectrum of indication, must be criticised when directly 
applied to the patient without the basis of empirical and 
experimental data from animals or animal studies.  The manifold 
appraised applications of ozone in man must be examined in controlled 
studies.  Otherwise, the ozone therapy will be regarded as an 
experimental or empirical therapy with little or no prospects of 
being accepted world-wide as a legal medical therapy.  Nonetheless, 
some forms of application with ozone have been examined in animals, a 
first step along the way.  The aforementioned Congress in Havana 
reported of the rectal and intra peritoneal application of Ozone in 
rats and rams and of the auto-transfusion with ozonised blood (ATOB) 
in horses; interesting aspects from the veterinary point of view.  
However, the true value of ozone therapy can only be estimated in 
connection with an induced disease (animal model).

Therefore, I would like to present you some results from animal 
studies where the application of ozone in induced lesions have 
comparative pathoetiogeneses to humans.

Publish Date: 1994