OxyFile #277

Lipid peroxidation and activity of antioxidative enzymes in the 
rat model of ozone therapy. 

Laszczyca P; Kawka Serwecinska E; Witas I; DoleÁych B; Falkus B; 
Mekail A; Zió...kowska B; Madej P; Migula P 

Department of Human and Animal Physiology, University of Silesia, 
Katowice, Poland. 

Mater Med Pol, 1996 Oct, 28:4, 155-60 


Hypothetical, therapeutic effects of ozone were investigated in an 
animal model. One ml of oxygen or mixture of 40 micrograms ozone 
with oxygen were injected intraperitoneally to male rats for 10 
days. Previously, rats had been poisoned with 50 ppm Cd2+ in 
drinking water for 12 weeks. Exhaustive treadmill running was 
applied to some animals before sacrification. Ozone injections 
increased iron-ascorbate-stimulated lipid peroxidation (LPO) in 
the liver and kidney, catalase (CAT) activity in the heart and 
glutathione S-transferase (GST) activity in the heart, kidney and 
liver. Oxygen increased GST activity in the brain and reduced 
glutathione peroxidase (GPX) activity in the kidney. Cadmium 
enhanced LPO in the liver and GST activity in the brain, heart, 
kidney and liver. In contrast to ozone, cadmium inhibited GPX 
activity in the brain, kidney and liver. Cadmium combined with 
ozone enhanced the changes of GPX activity in the kidney and 
liver, that of GST activity in the heart, kidney and liver as well 
as of CAT activity and LPO in kidney. The results suggest that 
ozone injections combined with tested factors may provoke an 
oxidative stress. The effects of ozone therapy can not be 
explained as the results of ozone action on the antioxidative 
enzymes in rat.