OxyFile #267

Effect of combined heat, ozonation and ultraviolet irradiation 
(VasoCare) on heat shock protein expression by peripheral blood 
leukocyte populations. 

Bulmer J; Bolton AE; Pockley AG 

Clinical Sciences Centre, University of Sheffield, UK. 

J Biol Regul Homeost Agents, 1997 Jul, 11:3, 104-10 


The re-administration of whole blood subjected to heat, ozonation 
and ultraviolet irradiation (VasoCare therapy) has been shown to 
elicit clinical benefits in individuals with vascular disease. 
Given that these stressors induce heat shock protein (Hsp) 
expression and that heat shock protein reactivity is implicated in 
the pathogenesis of vascular disease, this study assessed the 
effect of VasoCare on intracellular expression of Hsp60 and Hsp70 
by treated peripheral blood leukocytes. Contrary to expectations, 
VasoCare induced a significant reduction (approximately 40%) in 
the proportion of peripheral blood mononuclear cells expressing 
intracellular Hsp60 and Hsp70, whereas it had no effect on heat 
shock protein expression by peripheral blood neutrophils. Cell 
surface heat shock protein expression was not detectable. The 
reduced expression of Hsp60 by mononuclear cells was concomitant 
with an increase in the levels of Hsp60 in treated plasma. 
Although the mechanism underlying the clinical effectiveness of 
VasoCare therapy has yet to be established, it may be that re-
administration of treated blood or soluble factors derived 
therefrom modifies in vivo immune responsiveness to heat shock 
proteins or associated molecules.