OxyFile #247

Hydrogen peroxide in the regulation of erythropoietin (Epo) 
gene expression in hepatocellular carcinoma cells.

Author: Ndele JK; Yoshioka K; Fisher JW

Source: East Afr Med J 1996 Feb; 73(2):143-6


The present studies were designed and carried out to determine 
if hydrogen peroxide (H2O2) is involved in the regulation 
of erythropoietin (Epo) gene expression and stimulation 
of Epo production in the hepatocellular (Hep 3B) cells. 
Hep 3B cells were incubated with varying concentrati
ons of H2O2 for periods of 6 hours or 24 hours. In other 
experiments Hep 3B cells were incubated for 24 hours with or 
without increasing concentrations of catalase and in the presence 
of H2O2. Culture medium levels of Epo were determined and 
quantitation of Epo mRNA was also made. The results indicate 
that H2O2 increases the levels of Epo mRNA and Epo hormone 
production in Hep 3B cells, and that catalase, the specific 
scavenger of hydrogen peroxide, inhibits Epo production 
in these cells. Based on these findings, it is concluded 
that H2O2 takes part in the signal transduction mechanisms 
in Epo production. It is recommended that further studies 
be undertaken to find out the source of the hydrogen peroxide 
in the hepatocellular carcinoma cells.