OxyFile #243

Ginkgo biloba extract protects brain neurons against oxidative 
stress induced by hydrogen peroxide.


Author:   Oyama Y; Chikahisa L; Ueha T; Kanemaru K; Noda K

Source:  - Brain Res 1996 Mar 18; 712(2):349-52

Abstract:

Effect of Ginkgo biloba extract was examined on dissociated 
rat cerebellar neurons suffering from oxidative stress 
induced by hydrogen peroxide using a flow cytometer and 
ethidium bromide. Hydrogen peroxide at a concentration 
of 3 mM increased the number of neurons stained with ethidium 
(presumably dead neurons) in a time-dependent manner. Pretreatment 
of neurons with G. biloba extract (10 micrograms/ml) greatly 
delayed a time-dependent increase in number of dead neurons 
during exposure to hydrogen peroxide. It was true, but 
less effective, in the case of treatment with G. biloba 
extract immediately or 60 min after start of oxidative 
stress. Results implicate G. biloba extract as a potential 
agent in protecting the neurons suffering from oxidative 
stress induced by hydrogen peroxide.