OxyFile #232

In vitro detection of hydrogen peroxide in mesothelial cells.


Author:  Korybalska K; Breborowicz A; Martis L;Oreopoulos DG

Source:  Adv Perit Dial 1996; 12():7-10

Abstract:

Hydrogen peroxide can be indirectly detected in mesothelial 
cells by measuring the rate of inhibition of the intracellular 
catalase activity by 3-amino-1, 2,3-triazole (AT). AT binds 
and inhibits catalase only in the presence of hydrogen 
peroxide, and the effect is proportional to the amount 
of hydrogen peroxide. The effect of AT on catalase activity 
is prevented in the presence of ethanol. In mesothelial 
cells in vitro exposed to dialysis fluids (Dianeal 1.36%, 
2.27%, and 3.86%) no significantly increased generation 
of hydrogen peroxide was detected. However, interleukin-1 
(IL-1) (10 ng/mL) enhances, within two hours (+40%, p < 
0.05), intracellular production of hydrogen peroxide. The 
presented method of detection of intracellular hydrogen 
peroxide may be helpful in studies of the pathomechanisms 
causing mesothelial damage in conditions of peritoneal 
dialysis.