OxyFile #226

Topical hydrogen peroxide treatment of ischemic ulcers 
in the guinea pig: blood recruitment in multiple skin 
sites.


Author:  Tur E; Bolton L; Constantine BE

Source:  J Am Acad Dermatol 1995 Aug; 33(2 Pt 1):217-21

Abstract  

BACKGROUND: Oxygen deficit is a key factor associated with 
delayed healing of ischemic wounds in human beings. Topical 
oxygen-releasing compounds such as hydrogen peroxide or 
tetrachlorodecaoxide have been suggested as therapy for 
ischemic tissue. 

OBJECTIVE: Our purpose was to monitor the effect of hydrogen 
peroxide cream on the process of ischemic ulcer healing with a 
model for ischemic ulcers in the guinea pig. 

METHODS: Measurement of vascular perfusion with a laser Doppler 
velocimeter and gross observations of percentage of nonnecrotic 
wound surface were made on ischemic wounds in guinea pigs after 
treatment with either a hydrogen peroxide cream or a placebo 
cream. 

RESULTS: Visual evaluations of the percentage of nonnecrotic wound 
surface showed no statistically significant differences 
among the treatments. In contrast, vascular perfusion measurements 
resulted in statistically significant differences. Blood 
flow was significantly higher up to day 15 in ulcers treated 
with 2% hydrogen peroxide cream than in those treated with 
placebo cream. Vascular perfusion was significantly higher 
in ulcers treated with 3.5% hydrogen peroxide cream than 
in ulcers treated with either 1.5% hydrogen peroxide cream 
or placebo. Adjacent control sites in guinea pigs whose 
ulcers were treated with hydrogen peroxide cream showed 
increased vascular perfusion compared with corresponding 
sites in animals whose ulcers were treated with placebo. 
Even distant flank control sites of ulcers treated with 
3.5% hydrogen peroxide cream showed increased vascular 
perfusion. CONCLUSION: Treatment of ischemia-induced ulcers 
with hydrogen peroxide cream enhanced cutaneous blood recruitment 
not only to ulcers and adjacent sites, but also to distant 
sites.