OxyFile #195

UI  - 90298983
AU  - Sato H
AU  - Wananabe Y
AU  - Miyata H
TI  - Virucidal effect of ozone treatment of laboratory animal viruses.
AD  - Laboratory Animal Center for Biomedical Research, Nagasaki
      University School of Medicine, Japan.
AB  - An ozonization method was used to inactivate the viral pathogens
      of laboratory animals. Ozone at a concentration of over 100 ppm
      with high humidity was highly virucidal against 4 RNA viruses:
      HVJ, Theiler's murine encephalomyelitis virus (TMEV), Reo type 3
      virus (RV) and murine hepatitis virus (MHV). For the ozone tests,
      0.1 ml of a virus suspension in deionized water or saline and was
      placed in 35-mm dishes. The titer of 10(6) plaque-forming units
      of TMEV in a liquid-phase, which was highly stable against
      physical treatments, was reduced within 1 hr to a level of 0 by
      300 ppm of ozone at 80% humidity and 22-25 degrees C. HVJ and MHV
      were more susceptible than TMEV to the ozone treatment. RV was
      the most resistant of the 4 viruses. The ozonization method may
      be a good way to disinfect not only for the laboratory animal
      RNA-viruses (both of enveloped and unenveloped viruses) but also
      animal rooms, clean rooms and even safety cabinets.
LA  - Eng
SO  - Jikken Dobutsu 1990 Apr;39(2):223-9