OxyFile #184

UI  - 93312993
AU  - Bocci V
AU  - Luzzi E
AU  - Corradeschi F
AU  - Paulesu L
AU  - Di Stefano A
TI  - Studies on the biological effects of ozone: 3. An attempt to
      define conditions for optimal induction of cytokines.
AD  - Institute of General Physiology, Faculty of Pharmacy, University
      of Siena, Italy.
AB  - Ozonization of blood, normally carried out with citrated blood,
      may be fine for the autohemotherapy of ischemic diseases but it
      may be at a loss when employed in viral diseases or in
      immunodeficiencies. We have shown that heparin, used as an
      anticoagulant, with the addition of 5 mM CaCl2 favors production
      of cytokines by leukocytes with only a modest increase in
      hemolysis. High plasmatic levels of glucose, glutathione, and
      ascorbic acid decrease cytokine's yield because these compounds
      act as antioxidants and quench the inducing activity of ozone.
      Autohemotherapy with heparinized and Ca(2+)-supplemented blood
      has not revealed any side effects in volunteers.
LA  - Eng
SO  - Lymphokine Cytokine Res 1993 Apr;12(2):121-6