OxyFile #166

Human B Lymphocytes Show Greater Susceptibility to H2O2 Toxicity Than 
T Lymphocytes

Journal of Immunology, Vol. 132, No. 5, May 1994

C.M. Farber, L.F. Liebes, D.N. Kanganis, and R. Silber


Abstract:

Lymphocytes from patients with chronic lymphocytic leukemia (CLL) and 
from normal subjects were incubated with a glucose-glucose oxidase 
hydrogen peroxide (H2O2) generating system to study the effect of 
oxidant stress on these cells.  Within 4 hr, 90% of normal but only 
21% of CLL lymphocytes remained viable.  When normal and CLL 
preparations enriched in B or T cells were exposed to H2O2, B 
lymphocytes from both groups were highly susceptible to oxidative 
damage while T lymphocytes were relatively resistant.  The H2O2 
scavenger catalase prevented the cytotoxicity.  The present work 
identifies the human B lymphocyte as a cell that should be a suitable 
target for selective killing by H2O2 generating systems.