TI: Removal of Cholesterol and Other Lipids from Experimental Animal and Human Atheromatous Arteries by Dilute Hydrogen Peroxide. DT: June 23, 1982 AU: J.W. Finney, B.E. Jay, G.J. Race, H.C. Urschel, J.T. Mallams, G.A. Balla SO: Angiology 1966, Vol. 17, pp 223-228 AB: During the past decade, the volume and scope of experimental and clinical applications of oxygen at high pressure have increased at a rapid rate. An attempt has been made in this laboratory to obviate a number of problems inherent in the systemic administration of oxygen at high pressure by employing a regional system. In this system, hydrogen peroxide is used as a source of oxygen and the intra- arterial route as the means of administration. From these studies the following points have been made: hydrogen peroxide is broken down very rapidly when introduced into the blood stream in both rabbits and humans; in dilute solutions, intravascular hydrogen peroxide has no unacceptable deleterious effect on formed blood elements (with the exception of dogs, where, due to an apparent deficiency in RBC and plasma catalase, methemoglobin is produced); and the breakdown of hydrogen peroxide by biological fluids results in the supersaturation of these fluids with oxygen. The magnitude of the supersaturation is equivalent to several atmospheres of oxygen. Kann, Mengel, and others have shown that the formation of lipid peroxides is on sequela to exposure to oxygen at high pressure. The authors have noted a reduction in the subintimal lipid deposits and atheromatous plaques in the arteries of individuals being infused intra-arterially with hydrogen peroxide. This is a preliminary report on an extension of this finding approached in three different procedures: 1. Chemical and histologic evaluation of aortas taken at postmortem from patients who had been treated with intra-arterial hydrogen peroxide as an adjunct to external irradiation in the management of their malignant disease. 2. In vitro studies of human aortas incubated with hydrogen peroxide. 3. In vivo studies of the total scrum lipids in animals and humans before and immediately following the intra- arterial infusion of hydrogen peroxide.