TI: Studies on the Biological Effects of Ozone: 4. Cytokine production and glutathione levels in human erythrocytes. DT: December 14, 1993 AU: V. Bocci, E. Luzzi, E. Corradeschi, L. Paulesu, R. Rossi, E. Cardaioli, P. DiSimplicio SO: J Biol Regul Homeost Agents, 1993: 7: 133-8 AB: We have investigated the effect of various concentrations of ozone on human blood aiming to correlate the production of cytokines with depletion of reduced glutathione and hemolysis. As erythrocytes constitute the bulk of blood cells and represent the main target of ozone they have been taken as a useful marker of its oxidative activity. It appears that a transient exposure (30 sec) of blood of up to 78 ug ozone per ml of blood does not depress the production of cytokines even though there is a slight increase of hemolysis and a small decrease of intracellular reduced glutathione. In contrast either a constant (up to 30 sec) exposure of an ozone flux or a high ozone concentration (108 ug/ml) markedly decreases reduced glutathione levels and depresses cytokine production.