TI: Studies on the Biological Effect of Ozone 1. Induction of Interferon on Human Leucocytes DT: 1990 AU: Velio Bocci, Luana Paulesu SO: Haematologica 1990;75:10-5 AB: In this study we have investigated the effects of ozone on human blood, as well as on resuspended buffy coats and Ficoll-purified mononuclear cells. Samples were exposed at different ozone concentrations (from 2.2 ug to 108 ug/ml) for 30 sec and then incubated for different times at 37 Degrees C in a 95% air-5% CO2 humidified atmosphere. Supernatants were collected and frozen at -20 degrees C until tested for interferon (IFN) activity. We have determined that the ozone concentration is critical for lymphokine induction. In fact, while low concentrations (2.2 ug/ml) are effective in lymphocytes, they do not induce IFN in either whole or diluted (1:1) human blood, or resuspended buffy coats. In such cases levels as high as 42 ug/ml are required. On the other hand, a very high ozone concentrations (108 ug/ml) is not effective and probably toxic. Maximal IFN production occurs 72-96 h after ozone exposure, and the kinetics of IFN release is similar to that after Staphylococcal Enterotoxin B addition. Because ozonization of blood is a medical procedure followed in several countries for treatment of viral diseases, this study can open a new field of investigation that may yield useful results both in biological and practical terms.