TI The effect of ozone (03) on lymphocyte populations in normal and HIV1-infected blood. DT 9403 AU Wagner K; Mayers D; Toro L; Baker JR Jr; Henry M Jackson Foundation, Rockville, MD., USA SO Int Conf AIDS. 1989 Jun 4-9;5:656 (abstract no. C.587). Unique Identifier : AIDSLINE ICA5/00346989 AB OBJECTIVE: Measure the effect of varying 03 concentrations on lymphocytes in whole blood from an uninfected and a Walter Reed Stage 2 HIV1 patient. METHODS: Heparinized whole blood samples were exposed in triplicate to (03) of 20, 40, and 60 ug/ml with an oxygen control. Coded, blinded blood samples were gently agitated for 10 min. and incubated for 1 hr. at 27 C. Mononuclear cells were separated using Ficoll-hypaque gradients and stained for FACS analysis using labelled monoclonal antibodies. RESULTS: 03 had no effect on lymphocyte populations of the uninfected donor as numbers of total T cells, CD4, CD8, and B cell subpopulations did not change. In contrast, there were marked changes in the lymphocyte populations of the HIV positive donor with increasing (03): TABULAR DATA, SEE ABSTRACT VOLUME. CONCLUSIONS: Ozone, at concentrations previously shown to inactivate HIV1, may alter lymphocyte surface markers in HIV1 infected patients. Further studies are indicated to examine this effect. DE Flow Cytometry Human HIV Infections/*IMMUNOLOGY HIV- 1/*DRUG EFFECTS Leukocyte Count Lymphocytes/*DRUG EFFECTS Ozone/*PHARMACOLOGY MEETING ABSTRACT SOURCE: National Library of Medicine. NOTICE: This material may be protected by Copyright Law (Title 17, U.S.Code).