TI The Influence of Ozone on Tumor Tissue In Comparison With Healthy Tissue (in vitro) DT 0789 AU J. Washuttl, R. Viebahn and I. Steiner SO Ozone Science & Engineering. Volume 12, pp. 65-72 AB In comparison with healthy tissue, freshly operated carcinomatous ovarial tissue was treated in vitro with ozone in a suitable medium using air as the reference. In an analogous test arrangement and for reference purposes, the cytostatics Adriblastin (= Doxorubicin, an adriamycin) and Holaxan (= Ifosfamide, a cyclophosphamide) were included for the tumor tissue. After preparation of the tissue samples, the following substances were examined: 2,3-DPG, NAD (Nadide, DPN), Lecithin, Lysolecithin plus different enzymes and metabolites both in the mitochondrial fraction as well as, in the supernatant layer, the following: LDH, HBDH, Aldolase, ICDH, F-6-PK, cytochrome oxidase, Na, K, Ca and peroxidic metabolites. Statistically significant changes were found in tumor tissue after the action of ozone in the mitochondrial fraction in the case of NAD and 2,3-DPG, and in lysolecithin were the supernatant substances were concerned; this only applied to supernatant NAD in the case of healthy tissue. In all other parameters examined, no form of statistical change was found whatsoever in either healthy or tumorous tissue.