TI Inactivation of Human Immunodeficiency Virus Type 1 by Ozone in Vitro DT 9105 AU Keith H. Wells, Joseph Latino, Jerrie Gavalchin, and Bernard J. Poiesz SO Blood, Vol. 78, No. 7 (October 1), 1991:pp 1882-1890 AB A device was designed to deliver a constant source of given concentrations of ozone to fluids containing human Immunodeficiency virus type 1 (HIV-1). Ozone was found to inactivate HIV-1 virions in a dose-dependent manner. Greater than 11 log inactivation was achieved within 2 hours at a concentration of 1,200 ppm ozone. Similar concentrations of ozone had minimal effect on factor VIII activity in both plasma and immunoaffinity-purified preparations of factor VIII treated for the same time period. The data indicate that the antiviral effects of ozone include viral particle disruption, reverse transcriptase inactivation, and/or perturbation of the ability of the virus to bind to its receptor on target cells. Ozone treatment offers promise as a means to inactivate human retroviruses in human body fluids and blood product preparations.