OxyFile #15

 TI    Inactivation of Human Immunodeficiency Virus Type 1 by 
       Ozone in Vitro
 DT    9105
 AU    Keith H. Wells, Joseph Latino, Jerrie Gavalchin, and 
       Bernard J. Poiesz
 SO    Blood, Vol. 78, No. 7 (October 1), 1991:pp 1882-1890
 AB    A device was designed to deliver a constant source of 
       given concentrations of ozone to fluids containing human 
       Immunodeficiency virus type 1 (HIV-1).  Ozone was found to 
       inactivate HIV-1 virions in a dose-dependent manner.  
       Greater than 11 log inactivation was achieved within 2 
       hours at a concentration of 1,200 ppm ozone.  Similar 
       concentrations of ozone had minimal effect on factor VIII 
       activity in both plasma and immunoaffinity-purified 
       preparations of factor VIII treated for the same time 
       period.  The data indicate that the antiviral effects of 
       ozone include viral particle disruption, reverse 
       transcriptase inactivation, and/or perturbation of the 
       ability of the virus to bind to its receptor on target 
       cells.  Ozone treatment offers promise as a means to 
       inactivate human retroviruses in human body fluids and 
       blood product preparations.